Preferred Term:
chromatin immunoprecipitation
Definition:
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
Concept Schemes:
NALT Full
Broader Concept:
Entry Terms:
- chromatin immunoprecipitation sequencing
URI:
https://lod.nal.usda.gov/nalt/336371
Download this Concept:
RDF/XMLCreated 2020-02-19, last modified 2020-10-29