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NAL Agricultural Thesaurus
U.S. Department of Agriculture

Concept Breadcrumbs

Research, Technology, Methods > methodology > analytical methods > fluorometry > fluorescence recovery after photobleaching
Preferred Term:

fluorescence recovery after photobleaching

Definition:
A method used to study the lateral movement of membrane proteins and lipids. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data.
Concept Schemes:
NALT Full
Broader Concept:
fluorometry
Related Concepts:
Entry Terms:
  • FRAP (fluorescence recovery after photobleaching)
URI:
https://lod.nal.usda.gov/nalt/262830
Download this Concept:
RDF/XMLCreated 2015-10-08, last modified 2015-12-03