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fluorescence recovery after photobleaching

A method used to study the lateral movement of membrane proteins and lipids. A small area of a cell membrane is bleached by laser light and the amount of time necessary for unbleached fluorescent marker-tagged proteins to diffuse back into the bleached site is a measurement of the cell membrane's fluidity. The diffusion coefficient of a protein or lipid in the membrane can be calculated from the data.
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  • FRAP (fluorescence recovery after photobleaching)
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RDF/XMLCreated 2015-10-08, last modified 2015-12-03